Mock Orange - Poems by Anne Osbourn

Please join us in congratulating OpenPlant PI Prof Anne Osbourn on the publication of her book of poetry entitled Mock Orange:

MOCK ORANGE by ANNE OSBOURN  | SPM Publications, Poetry

Third Prize Winner, Sentinel Poetry Book Competition 2018

“Mock Orange is a collection of poems in which Anne Osbourn attempts to order her life and her origins and to try to understand how and why she became a scientist, specifically a plant biologist. From early childhood she has tried to make sense of the world through plants. In mid-eighteenth century Sweden Linnaeus, the father of modern taxonomy, spent his life trying to understand his Maker through the classification of plants. Osbourn’s poetry encompasses Linnaeus’s adventures and experiences and his fascination with living things. Mock Orange is therefore about journeys from origins, both personal and global, in which negotiations between scientific and non-scientific languages and points of view form a central theme.”

Post Doctoral Research Assistant, University of Edinburgh

Post Doctoral Research Assistant  – Experiments of Topologically Active Polymers & DNA Origami
From June 2021 or soon after to June 2024 (3 years) & potentially extendible
Salary high-end UE07 ~ £41k/year

We are looking for an experienced and motivated PDRA with background in experimental soft matter, polymer physics and/or molecular biology. The ideal candidate should have direct expertise on DNA biophysics and/or rheology of complex fluids. 

The project involves developing and performing experiments on polymers that can change topology in time and that are based on DNA. It also involves experiments with DNA origami and super-resolution microscopy. The aim of the project is to understand the rheological properties of polymeric materials and complex fluids made of “topologically active” polymers and DNA origami. 

This position is for a senior PDRA, who is expected to be semi-independent, have supervision responsibilities on a day-to-day basis and to propose and lead side-projects.

More info: https://www.vacancies.ed.ac.uk/pls/corehrrecruit/erq_jobspec_version_4.jobspec?p_id=052998

Contact: Davide Michieletto, davide.michieletto@ed.ac.uk
Group website: www2.ph.ed.ac.uk/~dmichiel/eutopia.unitn.eu

Research Assistant/ Lab Manager position in Chemical Biotechnology, University of Edinburgh

3.5-year Research Assistant / Lab Manager position in Chemical Biotechnology (Wallace Lab - IQB3)

We are looking for a highly-motivated MSc/PhD-level Research Assistant to join our lab in IQB3 from Dec/Jan. The role will be primarily research based and will continue our work on the combined use of chemical and biological tools in microbes for the sustainable production of industrial small molecules. This will include the assembly and optimisation of biosynthetic pathways in bacteria, the screening of new biocompatible catalysts, and the analysis of novel metabolites using a variety of analytical techniques. The post will also include a significant supervisory role, including the management of day-to-day research activities in the group, organisation of lab strains, coordination of group meetings and seminars.

Applicants can contact Stephen directly (stephen.wallace@ed.ac.uk) including a CV and cover letter, or apply directly using the link below.

Closing Date: 12th October 2020

Link here: https://www.jobs.ac.uk/job/CBP647/research-assistant

Website: http://wallacelab.bio.ed.ac.uk 

Twitter: @Dr_StephenW and @Wallace_Lab

Research Associate in Protein Design and Synthetic Biology, University of Bristol

Research Associate in Protein Design and Synthetic Biology

With Dr Fabio Parmeggiani, University of Bristol  

The group of Dr Fabio Parmeggiani is seeking a talented and enthusiastic postdoc interested in protein design and synthetic biology. The project will be focused on designing custom modular protein architectures as robust and reliable scaffolds for the development of novel functional protein-based nanomaterials and multivalent ligands to modulate cell response.

The aim of the project is to develop a robust, fast and high throughput design platform to rapidly build new proteins and complexes. The researcher will be responsible for developing the experimental part of the pipeline (involving DNA assembly, protein expression and purification, validation of designs through biophysical and structural characterization) and spearhead applications in nanomaterials and cell biology. The position would be best suited to a researcher with a keen interest in applying de novo protein design in synthetic biology.  Essential skills for this role would include: design, construction and expression of synthetic genes in E. coli; biochemical and biophysical characterisation of proteins; strong structural biology expertise, in particular with cryo-electron microscopy and x-ray crystallography.

Closing date: 14 October 2020 

More information can be found here.

Prof Anne Osbourn has been awarded an Order of the British Empire for services to plant science

We are excited to announce that Professor Anne Osbourn, one of our OpenPlant directors, has been awarded an Order of the British Empire for services to plant science. Earlier this year, Professor Osbourn was elected as a Fellow of the Royal Society, the 30th FRS in the history of the John Innes Centre.

Please join us in congratulating her!

Read more about this topic here.

An efficient and reproducible Agrobacterium-mediated transformation method for hexaploid wheat (Triticum aestivum L.)

Dr Sadiye Hayta and Prof Wendy Harwood from the John Innes Centre published their work on an efficient and reproducible Agrobacterium-mediated transformation method for hexaploid wheat, which has important implications for synthetic biology approaches to crop improvements:

Hayta et al. (2019) Fig 6: Transgenic plants showing GUS expression

An efficient and reproducible Agrobacterium-mediated transformation method for hexaploid wheat (Triticum aestivum L.)

Sadiye Hayta, Mark A. Smedley, Selcen U. Demir, Robert Blundell, Alison Hinchliffe, Nicola Atkinson & Wendy A. Harwood

Plant Methods Volume 15, Article number: 121 (2019)

https://plantmethods.biomedcentral.com/articles/10.1186/s13007-019-0503-z

Abstract:

Background

Despite wheat being a worldwide staple, it is still considered the most difficult to transform out of the main cereal crops. Therefore, for the wheat research community, a freely available and effective wheat transformation system is still greatly needed.

Results

We have developed and optimised a reproducible Agrobacterium-mediated transformation system for the spring wheat cv ‘Fielder’ that yields transformation efficiencies of up to 25%. We report on some of the important factors that influence transformation efficiencies. In particular, these include donor plant health, stage of the donor material, pre-treatment by centrifugation, vector type and selection cassette. Transgene copy number data for independent plants regenerated from the same original immature embryo suggests that multiple transgenic events arise from single immature embryos, therefore, actual efficiencies might be even higher than those reported.

Conclusion

We reported here a high-throughput, highly efficient and repeatable transformation system for wheat and this system has been used successfully to introduce genes of interest, for RNAi, over-expression and for CRISPR–Cas9 based genome editing.

Combining Transient Expression and Cryo-EM to Obtain High-Resolution Structures of Luteovirid Particles

OpenPlant PI George Lomonossoff and colleagues published their work on combining transient expression and cryo-EM to obtain high-resolution structures of luteovirid particles:

Byrne et al. (2019) Graphical abstract

Combining Transient Expression and Cryo-EM to Obtain High-Resolution Structures of Luteovirid Particles

Matthew J. Byrne, John F.C.Steele, Emma L. Hesketh, Miriam Walden, Rebecca F. Thompson, George P. Lomonossoff, and Neil A. Ranson

Structure (2019) https://doi.org/10.1016/j.str.2019.09.010

Abstract:

The Luteoviridae are pathogenic plant viruses responsible for significant crop losses worldwide. They infect a wide range of food crops, including cereals, legumes, cucurbits, sugar beet, sugarcane, and potato and, as such, are a major threat to global food security. Viral replication is strictly limited to the plant vasculature, and this phloem limitation, coupled with the need for aphid transmission of virus particles, has made it difficult to generate virus in the quantities needed for high-resolution structural studies. Here, we exploit recent advances in heterologous expression in plants to produce sufficient quantities of virus-like particles for structural studies. We have determined their structures to high resolution by cryoelectron microscopy, providing the molecular-level insight required to rationally interrogate luteovirid capsid formation and aphid transmission, thereby providing a platform for the development of preventive agrochemicals for this important family of plant viruses.

Improving plant transient expression through the rational design of synthetic 5′ and 3′ untranslated regions

Dr. Heydrien Peyret, Prof. James Brown and OpenPlant PI Prof. George Lomonossoff published their work on improving plant transient expression through the rational design of synthetic 5' and 3' untranslated regions in expression vectors:

Peyret et al. (2019): “Fig 1: The Synth expression cassette.”

Improving plant transient expression through the rational design of synthetic 5′ and 3′ untranslated regions

Hadrien Peyret, James K. M. Brown & George P. Lomonossoff

Plant Methods Volume 15, Article number: 108 (2019)

https://plantmethods.biomedcentral.com/articles/10.1186/s13007-019-0494-9

Abstract:

Background

The growing field of plant molecular farming relies on expression vectors that allow high yields of recombinant proteins to be produced through transient gene expression. While numerous expression vectors currently exist for this purpose, there are very few examples of systematic efforts to improve upon these. Moreover, the current generation of expression systems makes use of naturally-occurring regulatory elements, typically selected from plant viruses, to maximise yields. This study aims to use rational design to generate synthetic sequences that can rival existing ones.

Results

In this work, we present the rational design of novel synthetic 5′ and 3′ untranslated regions (UTRs) which can be used in various combinations to modulate accumulation levels of transiently-expressed recombinant proteins. Using the pEAQ-HT expression vector as a point of comparison, we show that pre-existing expression systems can be improved by the deployment of rationally designed synthetic UTRs. Notably, we show that a suite of short, synthetic 5′UTRs behave as expression enhancers that outperform the HT 5′UTR present in the CPMV-HT expression system. Furthermore, we confirm the critical role played by the 3′UTR of cowpea mosaic virus RNA-2 in the performance of the CPMV-HT system. Finally, we use the knowledge obtained from these results to develop novel expression vectors (named pHRE and pHREAC) that equal or outperform pEAQ-HT in terms of recombinant protein yield. These new vectors are also domesticated for the use of certain Type IIS restriction enzymes, which allows for quicker cloning and straightforward assessment of different combinations of UTRs.

Conclusions

We have shown that it is possible to rationally design a suite of expression modulators in the form of synthetic UTRs. We have created novel expression vectors that allow very high levels of recombinant protein expression in a transient expression context. This will have important consequences for future efforts to develop ever-better plant transient overexpression vectors for research or industrial applications.

"Plants can tell time even without a brain"

James Locke and Mark Greenwood (University of Cambridge) recently published their work on the coordination of circadian rhythms between different plant organs in PLOS Biology. This research paper has now been featured in The Conversation.

The article describes how the circadian timing in different plant organs is influenced both by local organ-specific input, as well as by inter-organ communication, allowing the expression of clock proteins to move through the plant in spatial waves.

Read more about the topic using the links above.

Building a CO2-concentrating mechanism

A new blog for the PLOS Synbio Community, written by Steven Burgess (former PDRA in one of the OpenPlant labs), describes the research of Alistair McCormack and colleagues on reconstructing an algal CO2-concentrating mechanism (CCM) into higher plants.

The work is part of an international collaboration that aims to test predictions that increasing the CO2 in plant leaves, with a system adapted from algae, will enhance photosynthetic performance, and water and nutrient use efficiency.

View the blog by Steven Burgess and the paper in the Journal of Experimental Botany.

New publications from the Baulcombe lab

OpenPlant PI David Baulcombe and colleagues recently published two papers: (1) on the miRNA-Argonaute machinery in the unicellular green alga Chlamydomonas reinhardtii, and (2) on the application of miRNAs for regulation of synthetic gene systems in this organism:

Chung et al. (2019): “Figure 1: Structural features of Chlamydomonas Argonautes.”

Distinct roles of Argonaute in the green alga Chlamydomonas reveal evolutionary conserved mode of miRNA-mediated gene expression

Betty Y.-W. Chung, Adrian Valli, Michael J. Deery, Francisco J. Navarro, Katherine Brown, Silvia Hnatova, Julie Howard, Attila Molnar & David C. Baulcombe

Sci Rep. 2019; 9: 11091. doi: 10.1038/s41598-019-47415-x

https://www.nature.com/articles/s41598-019-47415-x.pdf

Abstract:

The unicellular green alga Chlamydomonas reinhardtii is evolutionarily divergent from higher plants, but has a fully functional silencing machinery including microRNA (miRNA)-mediated translation repression and mRNA turnover. However, distinct from the metazoan machinery, repression of gene expression is primarily associated with target sites within coding sequences instead of 3′UTRs. This feature indicates that the miRNA-Argonaute (AGO) machinery is ancient and the primary function is for post transcriptional gene repression and intermediate between the mechanisms in the rest of the plant and animal kingdoms. Here, we characterize AGO2 and 3 in Chlamydomonas, and show that cytoplasmically enriched Cr-AGO3 is responsible for endogenous miRNA-mediated gene repression. Under steady state, mid-log phase conditions, Cr-AGO3 binds predominantly miR-C89, which we previously identifed as the predominant miRNA with efects on both translation repression and mRNA turnover. In contrast, the paralogue Cr-AGO2 is nuclear enriched and exclusively binds to 21-nt siRNAs. Further analysis of the highly similar Cr-AGO2 and Cr-AGO 3 sequences (90% amino acid identity) revealed a glycine-arginine rich N-terminal extension of ~100 amino acids that, given previous work on unicellular protists, may associate AGO with the translation machinery. Phylogenetic analysis revealed that this glycine-arginine rich N-terminal extension is present outside the animal kingdom and is highly conserved, consistent with our previous proposal that miRNA-mediated CDS-targeting operates in this green alga.

Navarro and Baulcombe (2019): “Figure 1: Construction of a synthetic circuit to measure miRNA-dependent gene repression.”

miRNA-mediated regulation of synthetic gene circuits in the green alga Chlamydomonas reinhardtii

Francisco J. Navarro and David C. Baulcombe

ACS Synth Biol. 2019 February 15; 8(2): 358–370. doi:10.1021/acssynbio.8b00393.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6396871/pdf/emss-81902.pdf

Abstract:

microRNAs (miRNAs), small RNA molecules of 20–24 nts, have many features that make them useful tools for gene expression regulation — small size, flexible design, target predictability and action at a late stage of the gene expression pipeline. In addition, their role in fine-tuning gene expression can be harnessed to increase robustness of synthetic gene networks. In this work we apply a synthetic biology approach to characterize miRNA-mediated gene expression regulation in the unicellular green alga Chlamydomonas reinhardtii. This characterization is then used to build tools based on miRNAs, such as synthetic miRNAs, miRNA-responsive 3’UTRs, miRNA decoys and self-regulatory loops. These tools will facilitate the engineering of gene expression for new applications and improved traits in this alga.

Genome-wide transcription factor binding in leaves from C3 and C4 grasses

OpenPlant PI Julian Hibberd and colleagues published their work on the transcription factor binding repertoire associated with C3 and C4 photosynthesis:

Burgess et al. (2019): “Fig 6: Hyper-conserved cis-elements in grasses recruited into C4 photosynthesis.”

Genome-wide transcription factor binding in leaves from C3 and C4 grasses

Steven J Burgess, Ivan Reyna-Llorens, Sean Ross Stevenson, Pallavi Singh, Katja Jaeger, and Julian M Hibberd

Plant Cell. 2019. pii: tpc.00078.2019. doi: 10.1105/tpc.19.00078.

http://www.plantcell.org/content/plantcell/early/2019/08/19/tpc.19.00078.full.pdf

Abstract:

The majority of plants use C3 photosynthesis, but over sixty independent lineages of angiosperms have evolved the C4 pathway. In most C4 species, photosynthesis gene expression is compartmented between mesophyll and bundle sheath cells. We performed DNaseI-SEQ to identify genome-wide profiles of transcription factor binding in leaves of the C4 grasses Zea mays, Sorghum bicolor and Setaria italica as well as C3 Brachypodium distachyon. In C4 species, while bundle sheath strands and whole leaves shared similarity in the broad regions of DNA accessible to transcription factors, the short sequences bound varied. Transcription factor binding was prevalent in gene bodies as well as promoters, and many of these sites could represent duons that impact gene regulation in addition to amino acid sequence. Although globally there was little correlation between any individual DNaseI footprint and cell-specific gene expression, within individual species transcription factor binding to the same motifs in multiple genes provided evidence for shared mechanisms governing C4 photosynthesis gene expression. Furthermore, interspecific comparisons identified a small number of highly conserved transcription factor binding sites associated with leaves from species that diverged around 60 million years ago. These data therefore provide insight into the architecture associated with C4 photosynthesis gene expression in particular and characteristics of transcription factor binding in cereal crops in general.

Joint OpenPlant Fund/ Biomaker call opens Monday 8 April and closes Monday 13 May

The next call for OpenPlant Fund applications is announced!

This year’s OpenPlant Fund call is joined with the Cambridge-Norwich Biomaker Challenge. More information on this joint call can be found at: https://www.biomaker.org/cambridge-norwich-challenge.

A summary of the important dates can be found below:

Call Opens: Monday 8 April

Mixer event in Cambridge: Thursday 25 April (Transport Norwich - Cambridge can be provided)

Call closes: Monday 13 May

Challenge Begins: Friday 24 May

Progress reports and presentations: Monday 29 July (OpenPlant Forum Event)

Challenge Closes/Open Technology Workshop: Saturday 2 November

Cambridge Science Festival

For this year's Cambridge Science Festival, Alex Ting (Cambridge OpenPlant coordinator) teamed up with Biomakespace, SciArt in Cambridge, and independent events producer Sophie Weeks to host The Art & Science Soirée. The event brought together scientists, engineers, artists and designers engaged in DIY science for an exciting evening of speed meets, snap-talks, hands-on demos, and unexpected encounters. 

The event opened with slam poetry by Peter Bickerton (Science Communicator, Earlham Institute) followed by a talk by Jim Ajioka (Co-founder, Colorifix) and Giulia Tomasello (Interaction Designer specialising in women's healthcare.) Inside the house, Biomaker Challenge teams exhibited their low-cost, open-source projects. The aim of the event was to provide inspiration for open science projects (talks and demos), showcase the tools available to pursue such projects (Biomaker Challenge), and highlight a community-access space for biology and prototyping (Biomakespace). We hope that the event will inspire and provide an avenue for artists, designers, and other non-scientists to get involved in open science. 

Photos of the event can be found here: https://www.flickr.com/photos/synbiosri/albums/72157679436063798

New OpenPlant Programme Manager at the John Innes Centre in Norwich

Hi all,

Dieuwertje van der Does, OpenPlant Programme Manager

My name is Dieuwertje van der Does and since February this year I am replacing Colette Matthewman as OpenPlant Programme Manager at the John Innes Centre in Norwich.

Previously, I obtained my PhD in the Netherlands, and worked as postdoctoral fellow at the Sainsbury Laboratory in Norwich to study the plant immune system. Before joining OpenPlant I spent two years at the BecA-ILRI Hub in Nairobi, Kenya, where I was Programme Lead for the 2Blades Foundation to aid the implementation of biotechnological solutions to crop diseases in East Africa. I am very excited to be able to contribute to the OpenPlant mission and accelerate the adoption of synthetic biology innovations in the real world. I am looking forward to our work together!

The Biomaker Challenge Winners and ways to get involved

The 2018 Summer Biomaker Challenge was wrapped up in October with a showcase event, but it not all over. Biomaker activities are still going strong! Below is a summary of activities as well as a write up of the Biomaker Fayre and the winning teams….

---

Biomaker Activities

Winter Software Challenge (apply by 16 December 2018): Interested in programming? Low-cost hardware for science? Learning new skills with a team? We provide the hardware, you develop software nodes for integrating hardware with new graphical programming interface, XOD. More information at www.biomaker.org/apply-now - a quick, rolling application process so you can receive your kit and start playing ASAP!

Norwich Biomakers - An interdisciplinary network exploring the cross-over of biology with design, technology, engineering, electronics, software, art and much more. A place to learn about the latest technologies, share ideas and skills and shape projects. We meet up on a monthly basis.

Cambridge Synthetic Biology meetups - A clearing house for a wide variety of regular open meetings like Cafe Synthetique, Science Makers and the SRI Forums - with a particular focus on building tools and interdisciplinary research.

Cambridge Biomakespace - Scientists, engineers, students and entrepreneurs are developing the new Cambridge Biomakespace - an innovation space for building with biology in the historic MRC Laboratory of Molecular Biology building.

---

The Biomaker Fayre

On Saturday 29 October, over 100 attendees came together in the University of Cambridge Department of Engineering to showcase and celebrate open-source technologies in research and education. The day consisted of a morning of talks followed by the Biomaker Fayre, where this year's ten Biomaker Challenge teams exhibited their projects alongside industry leaders and independent makers.

We started the day with some inspiring talks: Paolo Bombelli & Alasdair Davies on open tools for animal conservation and the "Powered by Plants" project, Grey Christoforo on hacking 3D printers to create better solar cells, Helene Steiner on OpenCell and teaching the next generation of designers to work with scientists, Richard Hayler on citizen science and education with Raspberry Pi and Julian Stirling on open instrumentation for Africa.

After a coffee break and lunch, we headed upstairs for the Biomaker Fayre. There was a festive feel to the space- gold balloons marked each exhibit, 3D-printed trophies were on display to be given out at the end of day, and attendees filled the space, excited to get involved and try out some hands-on demos.

Exhibits covered everything from a cartesian coordinate robot for dispensing fruit fly food to a wearable biosensor for monitoring vaginal discharge and a temperature-controlled container for sample transportation. Among the exhibitors were the ten Biomaker Challenge teams. In June, each team were given a £1000 grant and four months to turn their ideas for open source and DIY research tools into a reality.

The Biomaker Challenge judges were very impressed by each one of the projects and ended up deliberating for over an hour. In the end, the 3D-printed trophies (low-cost and DIY of course) were presented to the following teams:

Best Technology

Dual-View Imaging in a Custom-Built Light Sheet Microscope

Stephanie Hohn, Hannah Sleath, Rashid Khashiev, Francesco Boselli, Karen Lee

"The large variety of Biomaker projects was very inspiring. We had a lot of fun during the challenge and the feedback from people in different fields was really helpful. It was great to get in touch with programmers, engineers and designers. We received a great confidence boost for future more technical projects."

Stephanie Hohn (University of Cambridge)

Best Biology

Spectre, Low-cost whole-cell biosensors for environmental and medical surveillance.

Feng Geng, Boon Lim, Xiaoyu Chen, Jimmy Chen

"The Biomaker Challenge has provided us a great opportunity to extend our research into real-world application. As most of us come from a biological background, we faced a lot of difficulties on assembling the electronics and programming our Arduino kit. With three months of perseverance and constant guidance from our advisor Tony, we managed to come up with a customised, miniaturised spectrophotometer which can be used in conjunction with our whole-cell biosensor. We received an Arduino kit and sufficient funding to get us through the proof-of-concept stage of our project and from here, we are planning to further develop and optimise our device into a start-up company. It is amazing to think that it all starts with a small Biomaker Challenge Summer Project!"

Boon Lim, University of Oxford

Maker Spirit

Wearable biosensor for monitoring vaginal discharge

Tommaso Busolo, Giulia Tomasello, Michael Calabrese, James Che

"We all really enjoyed the multidisciplinary nature of the challenge, working with people from all sorts of backgrounds. We feel we now have a much clearer, hands-on insight into how the more diverse a collaboration is, the more relevant, impactful and exciting the results of ideas brainstorming can be!"

Michael Calabrese, University of Cambridge

Biomaker Challenge and Open Technology Workshop aimed to show the value of open, low-cost and DIY technologies as convening points for interactions between biologists and engineers. They are also important educational tools for those who are interested in developing technical skills and have great potential for improving the quality of science and increasing productivity in the lab for lower costs. With the proliferation of digital designs for 3D-printing and easily available consumer electronics like Arduino which has a huge community of users and lots of online help, designing your instrumentation around your experiment rather than vice versa has never been more possible.

Check out more photos from the day!

The descriptions of all prototypes are available at www.hackster.io/biomaker. To find out more about the most recent and upcoming competitions go to www.biomaker.org/biomaker-challenge to be kept up to date​​​​​​ with developments.

---

Biomaker Challenge 2018 was funded by OpenPlant, a BBSRC/EPSRC Synthetic Biology Research Centre Grant BB/L014130/1. The Biomaker Challenge and Open Technology Workshop were coordinated by University of Cambridge's Synthetic Biology Strategic Research Initiative

[Closes 3 April 2017] Postdoctoral Researcher - Plant Synthetic Biology

Opportunity for an outstanding post-doctoral scientist to work on a collaborative project between the Patron Group at the Earlham Institute (EI) and the O'Connor group at The John Innes Centre (JIC). The project aims to improve plant production chassis for heterologous bioproduction of proteins (including vaccines) and metabolites and to contribute to our understanding of how the rich endogenous metabolism of plants detoxifies foreign molecules. The post-holder will have access to facilities at the Earlham DNA Foundry, interact with stakeholders at LeafSystems® and have the opportunity to work and collaborate with scientists in the Cambridge-Norwich OpenPlant Synthetic Biology Research Centre.

For details see: http://www.nature.com/naturejobs/science/jobs/606787-postdoctoral-researcher or http://www.earlham.ac.uk/postdoctoral-researcher-plant-synthetic-biology-0